Composite

Part:BBa_K3904004:Design

Designed by: Ita Čiutaitė   Group: iGEM21_Vilnius-Lithuania   (2021-09-28)


Bile-regulated VapXD kill-switch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 199
    Illegal BamHI site found at 397
    Illegal XhoI site found at 442
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 179
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Different promoters can be inserted to regulate toxin VapD expression by using NcoI and AvrII restriction sites. Overall construct has the same RBS before promoter regions, so production can be altered by changing promoter intensity.